FastQCFastQC Report
Fri 10 Feb 2017
ERR1148443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545896
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169340.6651489298855885No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA107830.423544402442205No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT100570.39502791944368504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97400.3825765074457087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73400.2883071421613452No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG46520.18272545304285798No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.16017936317901438No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40500.15907955391736348No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA39920.15680137758965804No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39530.15526950040378712No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT36690.14411429217847077No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC33760.13260557383333807No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC29460.11571564588655624No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29120.11438016321169443No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28700.11273044931921807No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC28590.11229838139499806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2600.041.1686172
ATGGGGG54600.035.1784745
CATGGGG85100.033.820724
GGGTTAG7500.032.532881
TGGGGGG43850.031.1907166
CGCATAG3200.027.895951
GTTAGAG10000.027.3542583
TATAACG2507.075869E-1026.1649422
GGTTAGA8900.025.3898832
CATGGGT29500.024.5926274
ACGCATA2251.9243635E-723.786317
TAACGCA3655.456968E-1222.808794
ACATGGG345550.022.371733
GTACATT11550.022.1557451
AGTACTT135250.021.67630612-13
TTAGAGC11850.021.5782994
GAGTACT127300.021.44173612-13
ATCACGC5000.021.4076793
GTATCAA374950.021.3317171
TCACGCA5300.021.3179194
Produced by FastQC (version 0.11.5)