FastQCFastQC Report
Fri 10 Feb 2017
ERR1148442.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148442.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666381
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207440.7779833414654544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121530.45578632611018455No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA106350.39885522736623163No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT97470.3655516597215477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93280.3498374763396529No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51430.19288316260879448No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG47940.1797942604601518No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42610.15980461906981785No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41080.1540665043742811No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA39440.14791584548494757No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38620.1448405160402808No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC36650.13745222456955702No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC34060.1277386840065242No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31200.11701253496780842No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30940.11603743050974334No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC27830.1043736810305804No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT27070.10152337569162095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2650.040.3895722
ACGCATA3050.037.042177
ATGGGGG60800.035.1102185
GGGTTAG11600.033.340721
CATGGGG94650.032.856684
AACGCAT3450.032.7474256
TGGGGGG49350.031.3277236
GTTAGAG13700.030.8162963
GGTTAGA12900.029.5007422
TAGAGCA14050.028.7789695
TTAGAGC14300.028.275844
CTATAGC7350.027.5064323
CGCATAG4500.025.106368
CATGGGT33900.024.2059444
ACTATAG9000.023.7849712
GTAAGTA21100.023.6722492
CACTATA9050.023.011191
TGTAAGT21250.022.9601421
GTGGTTA13000.022.8847561
GTACATT13050.022.7970751
Produced by FastQC (version 0.11.5)