FastQCFastQC Report
Fri 10 Feb 2017
ERR1148440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893600
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178030.9401668779045205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107590.568177017321504No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA96050.5072348964934515No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT88970.4698457963667089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79530.4199936628643853No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45920.24250105618926912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41310.21815589353612166No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG38240.20194338825517533No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37290.19692648922686942No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36310.19175116180819604No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT32210.170099281791297No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32050.16925433037600338No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31050.16397338403041825No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA28940.15283058724123363No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27910.14739121250528095No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC26900.14205745669623998No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT24950.13175961132234898No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19750.1042986903253063No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19370.10229193071398396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAT2900.028.7066386
CATGGGG54800.027.4538784
ATGGGGG28450.026.3361325
ACGCATA3451.8189894E-1224.1302157
CTAGTAC4750.022.5342253
TATCACG1602.6224545E-422.2994942
CATGGGT16150.022.0923794
ATGGGAC26400.022.0742455
GTACTAG4050.022.0358281
TGGGGGG22150.021.7452076
TAACGCA3057.05586E-921.4465074
ATTAACG2505.3116673E-721.4075153
TTAACGC2255.104037E-621.1432234
GTATAGA4251.8189894E-1220.9988481
GAGTACT146150.020.97459612-13
AACGCAG380800.020.8622826
GTATCAA386700.020.7400051
TAGTACT6050.020.6408544
AGTACTT151500.020.56754112-13
ATACGGG2408.9197165E-619.8217723
Produced by FastQC (version 0.11.5)