FastQCFastQC Report
Fri 10 Feb 2017
ERR1148439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220276
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203870.9182191763546513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118970.5358342836656343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89440.40283280096708696No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77330.34829003241038503No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68950.31054697704249384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49100.22114367763287085No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36610.16488941014540534No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC32560.1466484346991095No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31300.14097346456026189No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27440.1235882385793478No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25820.11629184840082944No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT25720.11584145394536535No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA25310.11399483667796255No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC23920.10773435374701162No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC22760.10250977806362813No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22280.10034788467740045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1400.055.2190632
ATGGGGG58700.036.9767275
ACGCATA2750.036.760397
AACGCAT2950.036.283946
GGGTTAG12350.035.1764871
CATGGGG90000.034.4906734
TGGGGGG47500.034.051736
TATAACG1659.482392E-932.436372
CGCATAG3350.030.1764418
GGTTAGA13800.029.7333412
GTTAGAG14150.028.5776273
TTAGAGC14750.027.8183124
CATGGGT28000.026.7600064
TCACGCA3500.025.4857224
GTACATT10600.025.2640651
TAGAGCA16750.025.206775
GTATCAA356900.025.1950071
AACGCAG362550.024.8325826
TAGATCG1200.001292452424.7777845
ACATGGG338550.024.2047233
Produced by FastQC (version 0.11.5)