FastQCFastQC Report
Fri 10 Feb 2017
ERR1148438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981724
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204601.0324343854139124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123740.6244058203866936No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA101720.5132904481148737No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT97330.491138019219629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90250.45541155075076045No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG48190.24317210671112627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47350.23893337316397237No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC41840.21112929953918913No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41230.20805117160613687No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40070.20219768242197197No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32370.16334262490639465No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT32070.16182879149669682No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA30280.1527962521521665No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30220.15249348547022695No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29860.15067688537858953No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC28600.14431878505785872No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25270.12751523421021294No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC21920.11061076113525396No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20150.10167914401803682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2450.050.966647
AACGCAT3200.039.0213366
ATGGGGG44200.034.7089275
CATGGGG75250.032.31924
TATCACG2252.0736479E-1029.0705972
TGGGGGG37050.028.8879766
CGCATAG4400.028.3791528
TAACGCA2950.028.2195324
GTAAGTA15500.026.4704422
CATGGGT17400.026.3139024
TGTAAGT16000.026.0352421
GGACCGT2701.7262209E-924.2248846
TGGGGGC14550.024.1113686
TATAACG2502.0239895E-823.7850322
GAGTACT158400.023.01171912-13
AACGCAG409050.022.996566
GTATCAA413950.022.98711
AGTACTT164250.022.97047612-13
ATATGTA10000.022.6134681
CATGGGC66550.021.7121074
Produced by FastQC (version 0.11.5)