FastQCFastQC Report
Fri 10 Feb 2017
ERR1148437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9301630
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355710.3824168452196013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154730.16634718861102837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138830.14925341042376444No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT116080.12479533157091821No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109160.11735577527809643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA564000.033.2148251
ATGGGGG195250.031.065255
CATGGGG291300.030.8453524
TGGGGGG151050.029.9983716
AACGCAG624250.029.8540946
TCAACGC650150.028.7564144
TATCAAC657050.028.4906312
ATCAACG652350.028.458893
CAACGCA661000.028.26645
ACGCAGA706400.026.3822447
ACATGGG710300.026.1454373
GTACATG751950.026.0998541
CGCAGAG721100.025.8114418
TACATGG761900.025.6391222
GCAGAGT769550.024.2404739
GTAAGTA71350.022.6694362
CATGGGT113400.021.6572234
GGGTTAG32850.021.5569821
AGTACTT276600.021.0367712-13
TGTAAGT76400.021.0303651
Produced by FastQC (version 0.11.5)