FastQCFastQC Report
Fri 10 Feb 2017
ERR1148436.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148436.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997685
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210470.7021084603619127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122930.41008311413640863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92450.3084046522566581No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77020.25693159888380535No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT73670.24575630861815032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48550.16195831116344778No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35640.11889174479640123No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC31230.10418039253624048No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG30700.10241236153898758No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30610.1021121298602088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA3100.036.448187
AACGCAT3800.031.2989926
TATCACG2500.030.923922
ATGGGGG64250.030.451875
CATGGGG99900.030.0619454
CATGGGT40950.029.0447254
TGGGGGG54300.027.050866
GTATCAA341150.027.0325871
GTGGTTA19850.026.3768831
AACGCAG352700.025.8813466
TACAACG4450.025.391292
ACAACGC4950.025.2293053
GGGTTAG12500.025.2270931
GTTAAGA26750.024.2322584
GTAAGTA23300.023.9918162
ACATGGG335550.023.8904233
TATCAAC387550.023.753872
ATCAACG385550.023.6919983
TCAACGC388400.023.625334
CAACGCA392000.023.3931925
Produced by FastQC (version 0.11.5)