FastQCFastQC Report
Fri 10 Feb 2017
ERR1148435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880979
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152890.812821408426144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92730.4929879599931738No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA51380.27315562800009996No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45790.24343706123247522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.23865231881908303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27500.14620046263142755No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24540.13046397647182664No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21920.11653505966839608No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC21350.11350472280658105No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21090.11212246388715665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18850.10021377165826945No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG43350.033.88365
CATGGGG67250.032.6298984
GGGTTAG8700.030.7747341
TGGGGGG36100.029.4860046
GGTTAGA8750.029.224162
GTTAGAG9950.028.687973
GTATCAA244000.028.2858491
AACGCAG246600.028.020976
CATGGGT27900.027.0695554
ATCAACG266050.025.9060863
TATCAAC268650.025.832452
TCAACGC269550.025.6579534
CAACGCA271700.025.4549185
ACGCAGA275050.025.187467
CGCAGAG277150.025.0180668
ACATGGG239900.024.912513
TTAGAGC10300.024.826354
ATATGTA10800.024.7907581
GAGTACT102100.024.60770412-13
GTACATG262950.023.9394151
Produced by FastQC (version 0.11.5)