FastQCFastQC Report
Fri 10 Feb 2017
ERR1148434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792346
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178760.640178545208939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104760.3751684067805351No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA100060.35833668177224454No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT89060.318943282816671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80210.28724950274786865No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG44140.15807496635445606No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42630.15266732704328187No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39090.13998981501576094No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT37590.13461798788545545No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37340.13372268336373788No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA37240.13336456155505083No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC31120.11144750686340446No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC29730.10646961372265472No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC28180.10091872568800571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2203.6379788E-1232.433572
CATGGGG78850.029.9381544
ATGGGGG47950.029.1417375
ACGCATA2654.7293724E-1126.9259837
GGGTTAG9750.026.855571
GTATAGG5050.025.9249291
TGGGGGG41150.024.2759156
GGTTAGA11050.023.6769962
CATGGGT33400.022.9656874
GTTAGAG11700.022.8698253
GTATCAA362800.022.5539041
GTACACT27200.022.3160611
GAGTACT138700.022.22841512-13
AACGCAG377650.021.5865966
ACATGGG334650.021.4107763
AGTACTT145050.020.8453612-13
GTAAGTA14650.020.6999242
GTTAAGA20550.020.543894
GTGGTTA14850.020.4376051
GGTTAAG19350.020.2814583
Produced by FastQC (version 0.11.5)