FastQCFastQC Report
Fri 10 Feb 2017
ERR1148433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665960
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125460.7530793056255852No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA93870.5634589065763884No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90110.5408893370789215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80810.48506566784316546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.3151936421042522No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40750.24460371197387692No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40300.2419025666882758No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39030.23427933443780163No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36680.22017335350188477No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT35880.21537131743859395No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC33950.20378640543590484No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31450.18878004273812096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29770.17869576700521023No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC28110.16873154217388173No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25600.15366515402530673No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25080.15054383058416768No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG18430.11062690580806263No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA18260.10960647314461332No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA16950.10174313909097459No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA1700.052.467157
AACGCAT2000.047.5702136
CATGGGG47850.027.0906664
ATGGGGG25200.026.1919335
GGGTTAG3250.025.6362761
CATGGGT13650.023.5237334
CGCATAG3553.6379788E-1223.4501068
TGGGGGG20500.023.2049836
TATGTAA6400.022.2985382
AGTACTC10850.021.9217595
GAGTACT98000.021.35805512-13
AGTACTT102500.021.11653512-13
GTAAGTA8700.020.5044042
GTATAGA3801.9281288E-1020.3596341
GTACTTT125150.020.019881
CATGGGC50550.019.879744
ATGGGTA7300.019.5494025
ATATGTA5800.019.4955671
ACATGGG275650.019.479373
ATGGGAC17450.019.423375
Produced by FastQC (version 0.11.5)