FastQCFastQC Report
Fri 10 Feb 2017
ERR1148432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2672383
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206740.773616655995791No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117840.44095475835611886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86480.32360630942495894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73890.27649479883684336No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65090.24356538714697706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.18799700492032764No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC40560.15177465206147472No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37610.14073581518816727No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35970.13459897028232853No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG30030.11237161739166877No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC29790.11147354252739969No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2400.042.1210562
GGGTTAG10600.032.5618361
ATGGGGG77050.032.4144175
CATGGGG108750.031.769364
TGGGGGG60250.030.2013236
ATATGTA17050.028.2714041
GTGGTTA17850.027.671111
GTATCAA324800.027.5011841
GTTAGAG13300.027.273433
TTAGAGC13300.026.8263244
GGTTAGA13250.026.4787642
AACGCAG338450.026.4601336
CATGGGT39150.025.6694474
TATGTAA18850.025.2371442
TGTAAGT28400.025.1448841
GCGTATC2401.301305E-824.7956491
GTAAGTA28550.024.7857692
TATCAAC363750.024.734182
TCAACGC367400.024.3751564
ACATGGG323900.024.3074073
Produced by FastQC (version 0.11.5)