FastQCFastQC Report
Fri 10 Feb 2017
ERR1148431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438263
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234250.6813033208919737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127360.3704195985007546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102970.2994826166584697No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA95920.2789780770115608No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT89600.2605967024628424No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57000.1657813843792636No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC48050.1397507985863792No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG45160.13134539155381658No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37900.11023007838551035No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37600.10935754478351423No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36810.10705987296492443No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36170.10519846794733272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG89000.035.006145
CATGGGG127900.033.470714
GGGTTAG12250.033.024391
TGGGGGG75650.031.5951276
ACGCATA3350.030.1722057
GGTTAGA14100.026.9875762
AACGCAT4100.026.1030736
GGTTAAG33400.025.8121683
GTTAGAG14850.025.6245653
GTATCAA400500.025.5943851
GTTAAGA35200.025.5057014
ACATGGG405100.024.848363
GTAAGTA33400.024.5660632
TAGAGCA16350.024.3646415
TATCACG2752.1391315E-923.7827992
GTACATG460700.023.7091771
AACGCAG431650.023.6092436
TACATGG459400.023.4903012
CATGGGT41250.023.3503844
TGTAAGT33600.023.194981
Produced by FastQC (version 0.11.5)