FastQCFastQC Report
Fri 10 Feb 2017
ERR1148430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2575692
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162730.631791378782867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96980.37652017399595916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72170.2801965452391047No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA62010.2407508351153787No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT56720.22021266517891114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39000.15141561956942057No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG28450.11045575325000039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2300.033.610087
ATGGGGG56800.033.396155
CATGGGG88200.031.552324
TATCACG2500.030.9212762
TATAACG2800.029.7319952
TGGGGGG47350.029.3866396
TAACGCA3050.027.2949494
GTTAAGA22000.025.6776314
GTATCAA289350.025.6453761
AACGCAT3101.2732926E-1124.9365126
GGTTAAG20650.024.7646643
GTGGTTA16100.024.763671
AACGCAG301100.024.5084086
ACATGGG286700.023.8519673
ATATGTA12850.023.6174551
GGGTTAG9800.023.074041
GTACATG320650.022.7894171
TACATGG321550.022.7647882
TCAACGC327700.022.6823254
TATCAAC327500.022.6780222
Produced by FastQC (version 0.11.5)