FastQCFastQC Report
Fri 10 Feb 2017
ERR1148429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814233
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119310.6576332808409945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71530.39427129811881934No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA65530.3611994710712461No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62950.3469785854407896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53310.2938431833176885No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31450.1733514934410299No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28670.15802821357565427No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT26780.1476105880556687No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26660.14694915151471724No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26440.1457365178563062No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA26110.1439175673686897No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC23380.12886988606204383No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20990.11569627495476051No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20720.11420804273761968No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20250.11161741628555978No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC19880.10957798695095944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA1500.047.5673267
TATAACG1703.2923708E-1034.9759752
TTAACGC1209.3424205E-734.684514
AACGCAT1902.910383E-1134.423726
TATCACG1408.118877E-833.976662
ATGGGGG39650.033.890975
CATGGGG62550.031.939694
TAACGCA2151.2187229E-1030.4209654
TGGGGGG32200.029.9142346
TCTAACG1203.7145466E-529.7295782
CGCATAG2651.3915269E-924.681168
GGGTTAG6850.024.3219011
GTATCAA254300.023.5152591
AACGCAG260200.023.0112886
CATGGGT23200.022.8097614
GAGTACT95550.022.49615512-13
ACATGGG240200.022.0558323
TGGGGGC14350.021.9605226
AGTACTT99650.021.7197512-13
TACATGG274000.021.3749162
Produced by FastQC (version 0.11.5)