FastQCFastQC Report
Fri 10 Feb 2017
ERR1148428.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148428.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914855
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201680.6919040569771052No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132140.4533330131344441No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125060.42904364024968655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116710.4003972753361659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89350.3065332580865944No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG57280.19651063260436624No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53210.18254767389801552No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC52940.18162138425410526No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51220.17572057615215853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46840.16069409970650342No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46750.16038533649186665No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC42270.14501578980772628No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39390.13513536693935033No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG37250.12779366383576543No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC36880.1265243039533699No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30770.10556271238191951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3900.038.1165282
ATGGGGG59450.031.2061845
CATGGGG88200.030.3376454
TGGGGGG45850.028.4015926
ATCACGC6250.023.7847143
GTACGTA2102.7890037E-622.6699941
TCACGCA6700.022.1872314
ACGCATA2755.5602868E-821.6224657
GAGTACT151150.021.34245712-13
GTAAGTA21950.021.1299272
TGTAAGT22400.020.9874551
AGTACTT156400.020.7591112-13
CATGGGT38050.020.1591854
ACATGGG404650.020.0434633
GGGTTAG8650.019.9509051
GTATCAA456450.019.7775781
GGTTAAG20200.019.4281063
AACGCAG467200.019.2308966
ACCGTCG9450.018.8767558
GTATCAC9250.018.6567941
Produced by FastQC (version 0.11.5)