FastQCFastQC Report
Fri 10 Feb 2017
ERR1148427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991737
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206140.6890311548107337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118640.39655892212450494No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA109130.3647713686062645No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT103940.34742358703321846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94850.317039900231872No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG52420.175215936427567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.17093748548084275No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG44390.14837534181647652No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44280.1480076624382424No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44100.1474060052738593No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42130.1408212018636665No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC38340.12815297601360012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34780.11625353431802328No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC34570.11555160095957633No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31200.10428724182640386No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30790.10291680050753124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3000.033.6945652
ACGCATA2650.031.4128327
GGGTTAG11250.031.2038081
ATGGGGG60150.030.0512755
CATGGGG98650.028.9313764
TGGGGGG48050.028.8328446
GTTAGAG11100.028.3908143
GGTTAGA11050.027.4435372
CATGGGT49350.025.6635914
TATAACG3051.0913936E-1125.344032
TTAGAGC13700.024.7388344
AACGCAT3451.8189894E-1224.1286986
ATATGTA16100.024.0212481
TAGAGCA14050.023.6993625
GTATCAA426550.023.1829761
GTGGTTA13650.023.1020931
TATGTAA16200.022.756682
AACGCAG438550.022.4525746
GAGTACT150850.021.54119712-13
AGTACTT157100.021.49795212-13
Produced by FastQC (version 0.11.5)