FastQCFastQC Report
Fri 10 Feb 2017
ERR1148426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences633437
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69111.0910319416137675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45290.7149882308737886No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34220.5402273627843022No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT26060.41140634348798694No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17940.28321679977645764No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG15580.24595974027409195No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC13770.2173854700625319No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13230.20886054966792275No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12610.19907267810374196No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11100.1752344747780758No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.15455364937633892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.15171200924480258No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA9320.1471338112551051No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8310.13118905273926215No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8190.12929462598490457No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA7920.1250321657876No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.11334986746906164No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA6530.10308838921629143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA1700.041.9755673
GGGTTAG1703.255991E-1035.004511
ATGGGGG14250.034.2186785
CAGCGTC2101.8189894E-1233.980222
TATAACG1051.488556E-533.980222
ATAACGC2650.033.6596533
CATGGGG23300.032.667684
TACATTG3100.032.6100542
GGTTAGA1659.462383E-932.4356652
GTTAAGA4900.031.553064
AATAACG1751.6783815E-830.58222
GTCGGGA1951.4279067E-930.495072
TGGGGGG11500.029.4741486
GTTAGAG2252.0736479E-1029.0719663
TAACGCC1653.421046E-728.8317014
GTCAACG1254.874908E-528.5636791
GCAGCGT2502.1827873E-1128.5636791
TAACGCA1903.7334758E-828.1678144
TTGTAGC2751.8189894E-1228.110916
CATGGGT7250.027.8872154
Produced by FastQC (version 0.11.5)