FastQCFastQC Report
Fri 10 Feb 2017
ERR1148424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457183
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180550.7347845072996191No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA108750.4425799787805792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107720.4383881867976459No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98880.402412030361597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80480.32752953280240016No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG49180.2001478929326794No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG45440.18492721136358178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44180.17979938816115854No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40600.16522985874474957No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39960.1626252501339949No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA38960.15855554917969072No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35550.1446778689255135No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31960.1300676424995615No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30760.12518400135439647No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29520.12013757217105929No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC27460.1117539882051927No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25900.10540525471647817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2350.043.015327
GGGTTAG8350.030.6419091
TAACGCA3550.030.150544
AACGCAT3550.030.1499256
TATCACG1852.8894647E-828.928222
ATGGGGG58100.028.7594875
GTTAGAG8900.028.7295883
CATGGGG87750.028.4611934
TTAGAGC10500.027.1833444
TGGGGGG46350.026.6842926
TATAACG2256.6011125E-926.4282512
TAGAGCA11150.025.5986635
ATATGTA13600.025.3759861
GGTTAGA9900.025.2269652
TATGTAA14550.024.5210572
CGCATAG4150.024.3580728
GTGGTTA13350.023.622641
CATGGGT25800.022.8174134
GTTAAGA18100.022.0113754
AGTACTT151300.021.32120512-13
Produced by FastQC (version 0.11.5)