FastQCFastQC Report
Fri 10 Feb 2017
ERR1148423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304856
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147740.6409944916298459No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA106560.4623282322192796No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT103250.44796724827928514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87310.3788089147434807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64300.27897621369838294No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG47150.20456809449267113No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44560.19333094995956362No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA43590.18912244409195195No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG43190.1873869777547925No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT39670.17211487398778924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37000.16053063618724986No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC34220.14846914514399165No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32160.13953149350762042No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31960.1386637603390407No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29310.1271662958553593No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25330.10989840580062268No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23180.10057027423839059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2153.6379788E-1233.1886252
CATGGGG75500.030.4009254
ACGCATA2550.030.3144457
ATGGGGG46250.030.0850375
TGGGGGG38400.029.7314746
AACGCAT2701.8189894E-1228.6303086
GGGTTAG7400.027.3427621
CTAACGC1200.001292940224.776233
TATAACG2901.4733814E-1024.6053562
TAACGCA3650.024.4368274
GGTTAGA8500.022.3860512
CATGGGT28200.021.9295964
GTTAGAG9100.020.9100483
GCGTATC2004.9050606E-520.828751
TAGAGCA9200.020.6827645
GTAAGTA16400.020.6670022
GAGTACT116100.020.59013212-13
TTAGAGC9850.020.525284
TATGTAA11600.020.5044652
GTATCAA377600.020.4410481
Produced by FastQC (version 0.11.5)