FastQCFastQC Report
Fri 10 Feb 2017
ERR1148422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524039
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207360.8215403961666203No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA131440.5207526508108631No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123140.4878688483022647No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT116550.46175990149122104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90270.35764106656038197No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG54220.21481443036339773No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51950.2058209084724919No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51760.20506814672831916No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT49860.19754052928659185No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49430.19583691060241146No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA47610.18862624547402002No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41680.16513215524799735No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC40050.15867425186377865No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37220.14746206377952162No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG33380.13224835273939903No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC33230.13165406715189423No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT31740.12575083031601333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30520.12091730753764106No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT25300.1002361690924744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1950.054.8920172
ACGCATA2400.037.1657337
AACGCAT2500.035.6791046
GTACATT12150.034.2815061
ATGGGGG67800.033.5923465
CATGGGG98500.032.4197274
ATATGTA15600.031.658591
TATGTAA15950.030.5720442
GGGTTAG10100.030.0460151
TGGGGGG56000.029.5202126
TACATTG15600.027.4460092
ATCACGC4200.026.9014433
ATGTAAG18950.025.7321413
CATGGGT36350.025.6842294
GTACACT44050.024.9898621
GGTTAAG19700.024.7524913
GGTTAGA11750.024.2926392
ATTGTAG15950.024.2339385
TTGTAGC15950.024.2334586
GTAAGTA23650.024.1385612
Produced by FastQC (version 0.11.5)