FastQCFastQC Report
Fri 10 Feb 2017
ERR1148421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348348
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180140.7670924411543775No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA104720.4459305009308671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103890.4423961014296008No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT96500.4109271709303731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.29603789557595384No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG47100.20056652591523913No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41660.1774013050876616No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40700.17331332494161855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38380.16343403958868108No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36540.15559874430876514No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT36090.15368250361530744No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC33590.14303672198498688No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28260.12033991554914347No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC26750.11390986344442988No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25650.10922571952708883No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT24370.10377507933236471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2050.052.2156142
GGGTTAG9150.032.5161361
ATGGGGG44350.032.3150755
ACGCATA2307.2759576E-1231.026677
CATGGGG73950.030.3973274
CTATAGC7550.028.35553
TAACGCA2957.2759576E-1226.2061424
ACTATAG8400.026.1941432
GTTAGAG10450.026.1772083
TGGGGGG39200.025.9413056
AACGCAT2809.458745E-1125.4861936
GTACGTT2251.9121762E-723.8018111
ATCACGC4600.023.2700023
CATGGGT31450.023.2576714
TTAGAGC11650.022.9703884
CACTATA9600.022.9340361
TATAGCT11150.022.9337654
TAGAGCA12000.022.7959845
TCACGCA5000.022.597764
GTACACT24500.022.3445571
Produced by FastQC (version 0.11.5)