FastQCFastQC Report
Fri 10 Feb 2017
ERR1148420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460399
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137280.9400170775247038No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87520.5992882766969848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86510.5923723585129818No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT81130.5555331111566085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60520.41440729554046535No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39160.2681458971144188No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36640.2508903388731436No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.24205713644010984No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33190.2272666579475883No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32570.22302124282473487No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32480.2224049728875465No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC27810.19042741059121512No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC27520.18844165190471918No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC26210.17947150059675473No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24440.16735152516538288No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT23050.1578335783576954No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA17350.11880314900243015No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA15700.10750486682064285No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15620.10695707132091982No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG14900.10202691182341264No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14720.10079437194903584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG1950.042.6920132
ACGCATA1953.8198777E-1133.5437247
TATAACG953.2984721E-431.296782
AACGCAT2151.2187229E-1030.423386
GGGTTAG4300.027.6727771
CATGGGG45700.026.8042874
ATGGGGG24100.026.6477135
AGTACTC13150.024.4186255
ATATGTA6450.023.0606461
TGGGGGG20400.022.7361896
GGTTAGA5050.022.3725472
CGGTTAA1350.002564638422.0236572
CGCATAG3005.829861E-921.8034238
TATGTAA6300.021.7090342
GTAAGTA8500.021.6868252
GGGCTTT29000.021.119937
GCTTTTG26850.021.0393629
AAGTACT17000.020.987254
CATGGGT16450.020.9659884
GAGTACT110500.020.79890412-13
Produced by FastQC (version 0.11.5)