FastQCFastQC Report
Fri 10 Feb 2017
ERR1148418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386095
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139120.5830446817917978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82010.3436996431407802No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79320.3324259930975087No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71220.2984793145285498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65250.2734593551388356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.1500359373788554No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33720.1413187655981845No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31350.1313862189057854No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31100.13033848191291628No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30420.12748863729231233No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA28310.1186457370724971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2050.037.7095347
ATGGGGG42100.032.063095
CATGGGG68700.029.9482844
TGGGGGG34600.028.0138746
GTGGTTA14250.027.9730681
GTTAAGA17750.027.4705964
GGTTAAG18050.026.6845823
AACGCAT2905.456968E-1226.656746
TATCACG2251.924891E-723.7855172
GGGTTAG9700.022.6939541
TACAACG3307.4396667E-1021.6231962
GTATCAA291600.021.6067091
GAGTACT108050.021.51858312-13
GTTAGAG11150.021.3323023
AACGCAG293050.021.1237346
ACATGGG266550.020.8143
CATGGGT24850.020.5790184
AGTACTT113200.020.53960212-13
TAGAGCA11200.020.1756365
CGCATAG3251.4662874E-820.1266278
Produced by FastQC (version 0.11.5)