FastQCFastQC Report
Fri 10 Feb 2017
ERR1148417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049036
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142830.6970594952943726No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA105390.5143394259544488No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98440.480421037014479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86310.4212224675408338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67510.3294720053722824No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG44590.21761452702636752No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42710.20843948080951236No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT39620.19335921867648984No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37600.1835009243371029No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37420.18262246246527636No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA36640.17881579435402795No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC35750.17447228843221885No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31680.15460928944147392No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30490.14880168039995392No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29590.14440937104082113No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT27090.13220851170989675No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC21880.1067819208642503No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1452.6848284E-936.9106062
ATGGGGG48000.032.5830045
TAACGCA3200.031.5918884
CATGGGG73250.030.5250824
CGCATAG3650.029.344851
TGGGGGG38550.029.155076
ACCGTAG1505.31988E-627.7513053
GGGTTAG6550.027.2541221
ATATGTA11050.025.3097481
GTTAAGA16500.025.2284584
ACGCATA1951.4728193E-624.396757
TACAACG3002.2555469E-1023.7868332
AAGAGCG7000.023.786837
TATGTAA11550.023.6838592
AGTACTC14000.023.3620665
TATCACG1552.1149598E-423.0195162
CATGGGT18100.022.9983184
AGAGCGC6850.022.5714478
GTGGTTA10850.022.4856991
GTAAGTA16100.022.1616442
Produced by FastQC (version 0.11.5)