FastQCFastQC Report
Fri 10 Feb 2017
ERR1148416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946144
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147710.7589880296627587No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA116820.6002639064735189No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT109230.5612637091602677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88450.45448846539618853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64030.3290095696926846No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG49010.25183131361297006No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT47630.2447403686469244No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG47380.2434557771675683No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC44340.22783514477859806No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41670.21411570777907493No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC38060.19556620681717282No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37020.19022230626305145No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32350.16622613742867948No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32160.16524984790436884No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG29480.15147902724567144No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT28000.14387424568788332No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24780.12732870743377675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20400.10482266471545784No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20070.1031270039627078No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA19820.1018424124833517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1650.043.247892
AGCGTAA705.4718694E-542.4756136
ACGCATA2500.035.6795127
AACGCAT2600.034.3072246
TATAACG2550.032.647922
CTATAGC4950.031.234593
ATGGGGG46900.029.4159455
ACTATAG5550.027.857882
CATGGGG69700.027.7279824
GGTTAGA8800.027.7056852
GGGTTAG8600.027.6827161
GTTAGAG8600.026.275613
CGCATAG3400.026.2349388
TGGGGGG38400.025.5517356
TCACGCA2901.4733814E-1024.606564
TAACGCA3151.6370905E-1124.5414664
GTAAGTA16350.024.0045642
TAGAGCA10750.023.7863435
ATATGTA11500.023.289591
TATAGCT8700.023.2395294
Produced by FastQC (version 0.11.5)