FastQCFastQC Report
Fri 10 Feb 2017
ERR1148415.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148415.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312949
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170560.7374135789418617No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA127400.5508119720754759No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT118930.5141920552506779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101290.4379257821940734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77580.3354159559938416No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55720.24090457679784555No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52230.2258156146114765No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG51880.2243023949079725No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA49660.2147042585028896No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49460.21383956152945868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43670.1888065841486345No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43570.18837423566191905No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC38350.16580564465537287No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC35450.15326753854062497No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG34100.14743083396996648No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30380.13134747026415197No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23440.10134248528610013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23360.10099660649672776No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA23130.10000220497728225No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2200.048.6542787
AACGCAT3200.035.308146
CGCATAG3350.031.9520658
TATCACG2056.91216E-1131.908772
GGGTTAG7550.029.9585761
ATGGGGG51150.028.5996485
TACAACG2353.43789E-1027.8353062
GTTAGAG9950.026.8943233
CATGGGG80800.026.6420974
TTAGAGC10550.025.364794
TAGAGCA12000.025.2731975
GGTTAGA8750.025.1457672
ACCGTAG1451.3458075E-424.6067623
TGGGGGG40350.024.3170916
TATGTAA11050.024.2170622
TATAACG2352.9271723E-722.7743422
ATATGTA11250.022.7511
GAGTACT138200.021.90280512-13
AGTACAC23450.021.5549648
GTAAGTA14450.021.399652
Produced by FastQC (version 0.11.5)