FastQCFastQC Report
Fri 10 Feb 2017
ERR1148414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351301
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150200.6387952882255398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92020.39135780574243795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70940.3017053112298255No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46110.1961041993347513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39680.1687576367296233No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38960.16569550219219062No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37170.15808269549496215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24320.10343209993105944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2250.042.2880862
ATGGGGG68800.033.6226925
CATGGGG96200.031.8349024
TGGGGGG57150.031.4240446
GGGTTAG10600.029.747731
TATAACG2201.6007107E-1029.7338122
GTATCAA222950.028.6602921
ACGCATA1755.732927E-727.184627
GTAAGTA23850.026.6793942
AACGCAG243550.026.0523956
TAGAGCA13300.025.9326975
TATGTAA15450.025.788552
ATATGTA14250.025.4682311
ACATGGG243650.025.3095053
TGTAAGT24950.025.2766251
TTAGAGC12600.025.0136284
GTACATG266550.024.8427851
AACGCAT2401.3111276E-824.7776496
GTTAGAG12050.024.6748393
TACATGG268250.024.5186182
Produced by FastQC (version 0.11.5)