FastQCFastQC Report
Fri 10 Feb 2017
ERR1148413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461615
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109810.7512922349592746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73200.5008158783263719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49370.33777704799143415No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA37370.2556760843313732No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34700.23740861991700962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28970.1982054097693305No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16470.11268357262343366No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16210.110904718410799No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT14660.1003000106047078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA1156.6910616E-736.195287
TACAACG1802.2117092E-829.7328532
AAGAGCG4550.028.7516657
CTATAGC3550.028.4765363
ATGGGGG29400.027.1034165
CATGGGT18050.027.014894
CATGGGG45300.026.2541754
GTGGTTA10150.025.2173861
GTTAAGA12650.024.4444034
GTATCAA195050.023.8953511
AACGCAT1751.7594732E-523.785476
AACGCAG195950.023.4880756
ATATGTA7150.023.3103961
TGGGGGG24250.023.2950446
CACTATA4150.022.9493051
CGCATAG2102.805824E-622.6528268
ACATGGG178250.022.3517653
GAGTACT85950.021.89674412-13
GGTTAAG12800.021.8350643
TACATGG197550.021.703252
Produced by FastQC (version 0.11.5)