FastQCFastQC Report
Fri 10 Feb 2017
ERR1148412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930527
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78170.8400615995022176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52880.5682801251333922No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26860.2886536339085271No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC17390.18688334674867038No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17210.1849489590307428No Hit
GGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAG13180.1416401673460308No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12580.13519220828627218No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12570.13508474230194287No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12270.13186076277206357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12130.13035623899145324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.12766958938322048No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG11800.126809861508586No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT11110.11939470858986359No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10770.11574086512266704No Hit
GGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAG10720.11520353520102049No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG9760.10488680070540674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG6450.046.134561
ACGCATA705.469793E-542.4754337
TAACGCA1002.235065E-741.6259234
GTTAGAG7500.041.229493
GGTTAGA7350.040.45282
TAGAGCA8800.039.193245
TTAGAGC8300.037.9720154
TATAACG957.500412E-637.5572282
ATGGGGG30800.034.5595635
GTTAAGA12550.034.115734
CATGGGG41950.034.0208474
TGGGGGG25000.033.3007436
GTGGTTA7850.032.5997961
AACGCAT953.2969963E-431.297696
GGTTAAG11450.030.1223183
AACGCAG121600.029.830616
GTACGGG800.00535570529.7567881
GTATCAA124850.029.029851
ATAACGC3300.028.831813
GTACACT14100.028.2795031
Produced by FastQC (version 0.11.5)