FastQCFastQC Report
Fri 10 Feb 2017
ERR1148411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891389
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137780.7284593491872904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86450.4570714961332651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62690.3314495325921849No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48660.25727124351468683No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42410.2242267455293438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34660.1832515680275184No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23590.12472315319587879No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG19770.10452635602723713No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18940.10013804669478357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA1852.1827873E-1135.355987
CATGGGG56150.028.2750514
GGGTTAG5300.028.0720161
ATGGGGG32750.027.7793465
CATGGGT19850.026.6606924
TATCACG1807.3348565E-726.42772
TATAACG1901.1753546E-625.036772
AACGCAT2901.4733814E-1024.60516
TGGGGGG26250.024.0114546
AACGCAG240550.023.8047076
GGTTAGA6050.023.5883622
GTATCAA241500.023.386141
GTTAGAG6950.023.1004753
CGCATAG2853.2268872E-922.9503738
ACATGGG221700.022.690633
ACAACGC3950.022.5806313
TACATGG247850.021.9040162
GTACATG248550.021.860821
GAGTACT106100.021.63285612-13
TCAACGC268300.021.3647734
Produced by FastQC (version 0.11.5)