FastQCFastQC Report
Fri 10 Feb 2017
ERR1148410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775571
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115450.6502133679813423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70600.3976185688998074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45860.25828311005304777No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33360.18788322179175038No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29250.1647357385314358No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC27850.1568509510461705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27440.15454183471119995No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA1900.053.2050867
AACGCAT2650.038.147046
CGCATAG2650.035.90318
ATGGGGG48900.033.684395
CATGGGG72350.030.9856534
GGGTTAG8350.030.6414221
GTATCAA177500.030.571971
TGGGGGG39650.029.2448396
AACGCAG184850.028.69486
GGTTAGA8950.027.240722
TATCAAC202150.026.9744992
ATCAACG200600.026.9161383
TCAACGC202700.026.6666184
CAACGCA203850.026.4286675
ATATGTA10450.026.191991
GTTAGAG9850.025.9591243
TTAGAGC10600.025.8053514
GTTAAGA12450.025.7918324
ACGCAGA209250.025.2351157
CGCAGAG211450.024.9725638
Produced by FastQC (version 0.11.5)