FastQCFastQC Report
Fri 10 Feb 2017
ERR1148409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119832
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130390.6150959132610508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79610.3755486283818718No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74000.34908426705512513No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT70810.33403590473207306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59240.2794561078425083No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33610.15855030021246969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.1550594575419184No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31920.15057797032972423No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31550.14883254899444862No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30940.14595496246872394No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28180.1329350627785598No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC24710.11656584106665055No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23960.11302782484649727No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22420.1057630982077825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG2650.035.9290581
TATCACG2307.2759576E-1231.0261552
TATAACG2500.030.9227352
CATGGGG65900.029.5078054
GGGTTAG8300.029.3952731
ATGGGGG43300.027.7420185
TACAACG3300.027.0303632
ATATGTA10900.026.7510811
GTATAGG4300.026.2940121
GTTAGAG9550.025.5302493
TGGGGGG35150.025.3764136
CATGGGT26750.024.4536384
ATCACGC3451.8189894E-1224.1314543
TATGTAA11100.024.1081622
TAACGCA3950.024.087824
GTATAAC6450.023.9875221
GGTTAGA9850.023.545232
GTAAGTA14250.023.369412
ATGTAAG12700.022.4756413
GTATCAA289250.022.4246081
Produced by FastQC (version 0.11.5)