FastQCFastQC Report
Fri 10 Feb 2017
ERR1148408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5570606
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT666001.1955611292559554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445490.7997155067150683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272840.48978513289218445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138170.24803405589984284No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT107300.1926181819356817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95380.17122015091356307No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA95360.1711842481769488No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC82790.14861937821486565No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG79700.1430724054079574No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62700.1125550792858084No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA564150.046.712611
GGTATCA337950.046.3224371
AACGCAT6950.041.0656976
ACGCATA6650.040.235897
TATCAAC680300.038.7198642
ATCAACG701750.037.442463
TCAACGC718250.036.6154254
CAACGCA725550.036.1650735
AACGCAG733100.035.4033056
ACGCAGA841600.030.740167
CGCAGAG846000.030.5591988
GCAGAGT875800.029.4922319
GAGTACT375900.028.60806312-13
ATGGGAC87400.027.1446485
TATGTAA53300.026.6625232
GTAAGTA80500.026.517292
ATATGTA54500.026.3113751
GTACTTT416550.026.10201314-15
TGTAAGT80850.025.9787181
AGTACTT395550.025.92412212-13
Produced by FastQC (version 0.11.5)