FastQCFastQC Report
Fri 10 Feb 2017
ERR1148407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118843
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155350.7331831570342872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95280.4496793769052261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73660.3476425577543971No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46770.22073367399094695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42560.20086433964196498No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39570.18675286465302054No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC31410.14824128073670395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27180.1282775552506722No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22560.10647320259216941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA1700.041.97517
TACAACG2900.036.9091452
TATAACG1754.4929038E-1033.9798472
TATCACG1659.484211E-932.4353072
ATGGGGG59950.031.9393565
TAACGCA2850.031.2972284
GTTAGAG10350.030.450543
CATGGGG87750.030.3592034
GGGTTAG10400.030.3248941
GTTAAGA21900.030.139664
GGTTAGA10500.030.0155322
GGTTAAG21450.029.1086083
TGGGGGG48600.028.9982346
GTGGTTA15150.028.6725961
GTATCAA241900.028.4858681
TTAGAGC11250.028.0144944
AGAGCGC8100.027.8970348
AACGCAT2603.8198777E-1127.4452616
AACGCAG254350.026.7223056
GCGGTTA6250.026.6584451
Produced by FastQC (version 0.11.5)