FastQCFastQC Report
Fri 10 Feb 2017
ERR1148406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529613
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103830.6787991472352811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67270.43978444220858476No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50370.32929897954580667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46540.3042599664098043No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45990.3006642856722583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.16625120210144656No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23370.15278374333900144No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20510.13408620350376205No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT19640.12839849033709835No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19190.12545656973365157No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16550.10819730219343063No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16220.106039893750903No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC16110.1053207576033938No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15440.10094056470492863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG800.00537727929.7335412
CATGGGG43900.026.6856844
ATGGGGG26400.026.354735
TGGGGGG21000.024.919546
TATCACG1300.002059081622.8719562
CGACGGT3950.022.5824367
GAGTACT84200.022.24718912-13
CATGGGT13750.021.6243934
AACGCAG212450.021.1332786
GTATCAA212850.020.9403061
AGTACTT86850.020.37012912-13
GGGTTAG3801.9463187E-1020.3579771
ACATCGC2659.292453E-720.1963678
GTACTTT106200.020.0601121
GTACCGT1500.00472168919.8223616
TACGACG4203.45608E-1119.8223615
AACGCAT1500.00472168919.8223616
ATATGTA6400.019.526041
ACATGGG191400.019.5116653
ACACCGT3051.6556623E-719.4974046
Produced by FastQC (version 0.11.5)