FastQCFastQC Report
Fri 10 Feb 2017
ERR1148405.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148405.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447527
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110830.7656506579842725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71060.49090621453002253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51540.3560555347154147No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43830.30279227952224724No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36890.25484844151438973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28440.19647301915611937No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19450.13436709643412523No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18140.12531717888509158No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT16490.11391842777371337No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC15130.10452309352433495No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14860.10265784334247306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1652.382876E-1036.037232
ATGGGGG31000.031.6488275
TATCACG1203.7130623E-529.7307172
CATGGGG47300.029.0393034
TAACGCA2103.1614036E-928.3149684
TGGGGGG25600.028.1048186
CTATAGC3700.027.320123
GGGTTAG4150.025.8101161
CATGGGT19500.024.3944344
GTATCAA195300.023.8270381
AACGCAG199050.023.5097476
ACATGGG178950.022.329193
GCGTATC1602.60974E-422.3149971
CGCATAG1653.2134022E-421.6387831
ATCAACG215700.021.4744833
TCAACGC217750.021.4634634
AGTACTT88500.021.36580512-13
CAACGCA220400.021.3133075
TATCAAC217750.021.299622
GAGTACT85900.021.11261612-13
Produced by FastQC (version 0.11.5)