FastQCFastQC Report
Fri 10 Feb 2017
ERR1148404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247004
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173630.77271780557578No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109400.4868705173644551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78640.34997712509635054No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70180.312326991852262No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59390.2643074956697896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44930.199955140266773No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG29220.13003982191397967No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG28680.12763662191967615No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28460.12665754044051547No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26750.11904740712522097No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26030.11584314046614959No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23570.10489522938098908No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23140.10298157012626592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA2300.036.1967287
AACGCAT2400.034.688536
GGGTTAG12950.033.0842931
ATGGGGG60100.033.0484285
CATGGGG88550.031.0264594
TGGGGGG49900.029.7926126
GGTTAGA14750.028.6256122
ATATGTA15300.028.3916451
CGCATAG3150.028.3171678
GTTAGAG14950.028.2420313
TATGTAA15250.028.0770262
TATAACG2154.0618033E-927.659862
GAGTACG1358.2817154E-526.4293586
TTAGAGC16350.026.187474
TAGAGCA16000.026.0169775
TATCACG1901.1744214E-625.0394542
GGTTAAG22100.025.0247333
CATGGGT30750.024.947294
GTATCAA316350.024.6600531
GTAAGTA22750.024.5716842
Produced by FastQC (version 0.11.5)