FastQCFastQC Report
Fri 10 Feb 2017
ERR1148402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522969
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89080.5849101327735495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59070.3878608166023077No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50280.33014460570110093No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT47260.31031491776917325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43200.2836564631322108No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.1497075777642224No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21710.14255050496759947No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20950.13756025237545874No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20390.13388322414967083No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC18230.11970040099306026No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16700.10965423459046114No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC16000.10505794930822623No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15520.10190621082897945No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15420.10124959864580302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG47750.031.132964
ATGGGGG31500.030.3926875
TGGGGGG24250.029.9158846
GGGTTAG5250.026.0671351
CATGGGT16100.025.4845494
TATAACG1552.1153675E-423.0183032
GGTTAGA5500.022.7044182
GGTTAAG10250.022.0451723
GTAGCGT1350.002564669822.0236853
ACATGGG183600.021.9265233
TTAACGC1903.3220404E-521.9077724
GTAAGTA10700.021.6737772
GAGTACT78100.021.0141512-13
TACATGG211050.020.9060672
GTACATG211300.020.8098871
GTATCAA200650.020.7875651
AACGCAG203300.020.7670466
GTACCGT1754.8039932E-420.387646
TTAGAGC6500.020.126264
GTGTTAT5050.020.0300621
Produced by FastQC (version 0.11.5)