FastQCFastQC Report
Fri 10 Feb 2017
ERR1148399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771611
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63230.8194543623665294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43110.5587012108432876No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33220.4305278177734636No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28450.36870910342128355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18060.23405576125793956No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16030.20774716793824868No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG14360.18610413796589215No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13990.1813089756366874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13610.17638421432561224No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11300.14644684951355022No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10850.1406148953293823No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10020.12985817983413922No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9740.12622940834176807No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC9340.12104544906695212No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9280.12026785517572974No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9280.12026785517572974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG802.301469E-644.6008452
ACGCATA758.215445E-539.6426247
CGCATAG851.7160679E-434.9787868
ATGGGGG17850.033.3131335
ACCGTAG902.4007974E-433.035523
CATGGGG28400.032.6632924
CATGGGT11050.031.7499774
AGCGTCA1352.3402736E-630.8331533
GTATTAC1004.4345224E-429.7531781
TGGGGGG16100.028.8086176
AACGCAT1055.924849E-428.3161626
GGTTTCA2950.028.2403031
GGGTTAG2853.6379788E-1227.1432511
GTACAAC2455.529728E-1026.717141
ATTAACG1358.276802E-526.4284173
CCGTAGC1150.001008072325.8538884
ACAACGC1401.0598365E-425.4845473
TTAACGC1401.0598365E-425.4845474
AATAGGG1401.0598365E-425.4845477
GAGTACT49300.025.2691612-13
Produced by FastQC (version 0.11.5)