FastQCFastQC Report
Fri 10 Feb 2017
ERR1148398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037572
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97850.9430670835373353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63990.6167282848804709No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA59330.571815739052326No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51780.4990497045024345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.40729703577197535No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG26430.2547293103514744No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT24840.2394050726118284No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24720.2382485263673268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.2323694162911104No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC19980.19256494970951413No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC18410.17743346967728504No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC17960.1730964212604041No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17290.16663903806193692No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16240.15651925842254802No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG15740.15170031573712472No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT15580.1501582540777893No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15390.14832705585732844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12450.11999167286703959No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT12060.11623289757240944No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA11530.11112481832586076No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG10760.10370364659030892No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10600.10216158493097345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1101.7826096E-1048.6596372
ACGCATA1204.2018655E-1044.6025167
TACAACG1256.2573235E-1042.8204842
GGGTTAG5100.039.673451
AACGCAT1401.902663E-938.2307326
ATGGGGG31000.034.916345
TATAACG1251.2824185E-633.304822
TGGGGGG24550.033.186946
GTTAAGA8200.032.637564
GGTTAAG8300.032.244343
CATGGGG44400.032.013564
CGCATAG1501.4846046E-731.7173488
ATCACGC1701.2656528E-831.4856473
TAGAGCA7250.031.1719995
GGTTAGA6400.030.6657072
TTAGAGC6600.030.637554
GTTAGAG6500.030.1939283
TCACGCA1954.8044967E-827.4490284
GTGGTTA5900.027.233471
TATGTAA5700.027.1279832
Produced by FastQC (version 0.11.5)