FastQCFastQC Report
Fri 10 Feb 2017
ERR1148396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605853
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118800.7397937420174823No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA79390.4943789998212788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76800.4782504998900896No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71790.44705212743632206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50580.31497279016198865No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36920.2299089642700795No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT36530.22748034845032514No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34500.21483909174750118No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.1841388968977858No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC27430.17081264598939006No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23980.14932873681463996No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23700.14758511520045733No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21620.13463249749510073No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20880.1300243546576181No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20070.12498030641658982No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT19720.12280077939886154No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16560.10312276403880057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1850.038.570442
GGGTTAG6350.031.8512231
TATGTAA8050.030.2853812
ATGGGGG34100.029.9929475
ATATGTA8450.029.5674231
TGGGGGG28150.028.7280146
CATGGGG56600.028.4706864
ACGCATA1903.7425707E-828.166577
TATAACG1704.4507942E-727.9824732
TACAACG2803.6379788E-1227.6077122
ACAACGC3250.027.4443533
GTTAGAG7150.027.4443513
ATCACGC2905.456968E-1226.6557223
TAGAGCA7450.026.3392095
AACGCAT2057.820745E-826.1056026
GGTTAGA6900.025.8533742
GGTTTCA7550.025.2129651
TTAGAGC7200.024.776154
GTACAAC5650.024.2158831
GTTAAGA11900.023.984984
Produced by FastQC (version 0.11.5)