FastQCFastQC Report
Fri 10 Feb 2017
ERR1148394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768093
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123130.6964000196822225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74800.4230546696355904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57140.3231730457617331No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA57130.3231164876508193No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT46340.26209028597477624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33090.1871507890139263No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG25980.14693797215417967No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT23250.1314976078746989No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21770.12312700745944925No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC20450.11566133681882118No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20360.11515231382059653No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC18210.102992319974119No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2050.043.5138552
ATGGGGG38900.034.7029535
ACGCATA1701.2676537E-831.4835537
TGGGGGG32400.031.019296
CATGGGG61000.030.806864
TAACGCA2651.8189894E-1229.1734394
GGTTAGA7150.028.2789342
GGGTTAG7350.027.5327991
TATAACG1954.8115908E-827.44722
TCTAACG1107.776584E-427.0313362
GTTAGAG8000.026.017663
GTAAGTA12600.025.4866872
ATCACGC3800.025.0395533
AACGCAT2302.3771281E-723.2704526
TCACGCA3603.6379788E-1223.1268064
GTATTAC2853.1905074E-922.9724121
TAGAGCA9700.022.6840275
ACATGGG229450.021.7970683
TATAAGT4650.021.7413314
AACGCAG248900.021.479546
Produced by FastQC (version 0.11.5)