FastQCFastQC Report
Fri 10 Feb 2017
ERR1148392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683733
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135160.802740101904518No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA90920.5399906042110002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85720.5091068477009122No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74780.4441321753508425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65810.3908576953709406No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG39240.23305357797227944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37920.2252138551658725No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT36550.21707717316225317No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34910.20733691149368694No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31950.18975692701871377No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC27900.16570323204451062No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC27810.1651687054895283No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA26500.15738837452256385No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23760.14111501051532516No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22690.13476008369498013No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT22060.1310183978101041No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA18260.10844949882196286No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC17140.10179761280440544No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA17020.10108491073109573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2150.060.8460582
ATCACGC3650.037.468843
TCACGCA3500.037.375754
AACGCAT2650.035.9012376
ACGCATA2400.034.6858337
GGGTTAG7500.032.53651
ATGGGGG40350.032.125385
CATGGGG63000.031.3352284
TGGGGGG33450.030.397326
GGTTAGA8350.029.909632
GTTAGAG8300.029.372513
TACAACG2252.0736479E-1029.0708922
GTACATT7850.028.8112531
TTAGAGC9200.026.4991134
TAGAGCA10100.025.9039885
GTATCAC5600.025.5077081
CACGCAG4900.025.4834695
CATGGGT19850.024.8629634
CGCATAG3151.6370905E-1124.5629771
GTACACT25950.024.082421
Produced by FastQC (version 0.11.5)