FastQCFastQC Report
Fri 10 Feb 2017
ERR1148391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630628
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130570.8007344409638495No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA87690.5377682708747795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84130.5159361914550713No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71360.4376228054467359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64140.3933453859494624No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40740.24984239201093073No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37740.23144457227522158No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG35680.21881140272336794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.21549979517094028No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32270.19789921429044516No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA29310.17974669881787878No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC27080.16607098614766824No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC26790.16429253023988302No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24450.14994223084602987No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT23800.14595603656995956No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22290.13669580063631925No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16310.10002281329647227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1600.048.3167082
TATCACG1555.151378E-934.5290572
ACGCATA1352.3415323E-630.8345937
CATGGGG49250.029.3409374
ATGGGGG29000.029.1181855
TAACGCA2701.8189894E-1228.632124
AGTACTC12650.026.7952795
ATTAACG1807.3290903E-726.429653
TGGGGGG23200.025.6322046
GTTAAGA9950.024.5038724
GTGGTTA8000.023.8012831
AAGAGCG5900.023.181947
GTATAAC4400.022.9898761
TTCAACG10300.022.5165234
GGTTAAG9650.022.1844733
CTACGCA2704.571848E-822.0247084
TACCGTC5200.021.7282227
GGGTTAG4400.021.637531
ACCGTCG4950.021.6242588
CGCATAG2505.278289E-721.4211541
Produced by FastQC (version 0.11.5)