FastQCFastQC Report
Fri 10 Feb 2017
ERR1148390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423245
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94620.6648187768093337No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67980.4776408840361287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60760.4269117404241715No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT57400.40330371791223574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45210.31765437433470695No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG29870.2098725096522384No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28920.20319762233487557No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG28560.2006681913514539No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24170.16982318574806163No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23160.16272672660012857No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22020.15471686181929323No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21250.14930668999364127No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20290.1425615407045168No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19190.1348327238107283No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18950.13314643648844718No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT17610.12373133227237755No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15520.10904658017417944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1600.044.5959362
ACGCATA1850.041.783587
ATGGGGG33400.032.0449875
CATGGGG50450.031.1155014
CGCATAG2351.0913936E-1130.3631928
TGGGGGG27850.030.3177666
TCACGCA3000.029.7306254
GGGTTAG6100.029.2638021
AACGCAT2651.8189894E-1229.1696686
ATCACGC2950.028.2188993
ATGGGTA6100.025.344145
GTACATT6600.025.243721
CATGGGT14150.025.213254
GGTTAGA6850.025.1733762
TATGTAA7400.024.9094412
ATATGTA7200.024.792941
TACAACG2001.8367373E-623.78452
GTTAGAG7200.023.123823
TTAGAGC7550.022.0518534
GTTAAGA8550.021.559054
Produced by FastQC (version 0.11.5)