FastQCFastQC Report
Fri 10 Feb 2017
ERR1148387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462099
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215430.8749851244811846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126870.5152920333422824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95220.38674318132617735No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82190.33382085773155346No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT76950.31253820419081446No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49370.20051996284471096No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG44860.1822022591293039No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA35200.14296744363244532No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33300.13525045093637583No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31490.12789899999959384No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA31380.12745222673824244No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27660.11234316735435904No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC26840.10901267576973955No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT26230.10653511495679092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3100.044.127212
ACGCATA2550.030.3209977
ATGGGGG50750.028.0092955
TCACGCA5100.027.9886114
CATGGGG82800.027.0083354
GTACATT11250.025.3985211
AACGCAG407550.025.1737846
GTATCAA409300.025.0589731
TGGGGGG42300.024.4652256
ATCACGC5450.024.0085813
TACAACG3750.023.7903212
AACGCAT3302.910383E-1123.429866
CGCATAG3603.6379788E-1223.129488
ATATGTA11650.022.9935671
ACGCAGA449950.022.6429737
CGCAGAG452550.022.5128828
TATCAAC457250.022.4895942
ATCAACG456200.022.4109843
CATGGGT26550.022.4014324
AGTACTT175150.022.39468612-13
Produced by FastQC (version 0.11.5)