FastQCFastQC Report
Fri 10 Feb 2017
ERR1148386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4106266
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353060.8598079130772337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201200.4899828700819674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164900.4015813880542566No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125700.30611752867446973No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT114200.2781115495196853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81390.19820927333981772No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG62730.15276652803301102No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA52340.1274637346922971No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG47980.116845815638831No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC45270.11024614576844267No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA44600.10861449306985957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA4350.030.0793557
CATGGGG124600.029.260224
ATGGGGG70900.029.1084025
TATCACG4250.026.5878932
AACGCAT5900.026.2093626
CGCATAG5550.025.718968
AACGCAG597400.025.655736
GTATCAA603750.025.4291271
GGGTTAG12600.024.5680121
TGGGGGG60250.024.2836046
ACGCAGA661800.023.1142237
CGCAGAG665500.022.967848
TATCAAC671350.022.793452
TCAACGC679700.022.645514
ATCAACG673850.022.6126253
CAACGCA684450.022.5839375
GCAGAGT692050.022.0609159
GAGTACT261150.021.97720112-13
CATGGGT40700.021.7734384
AGTACTT274550.021.6302612-13
Produced by FastQC (version 0.11.5)