FastQCFastQC Report
Fri 10 Feb 2017
ERR1148385.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148385.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786448
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290710.7677644061135924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160960.42509497027293125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134240.3545275149691743No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74910.1978371286229205No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT65900.17404174043853235No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63010.16640925743599277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA429350.031.2007771
AACGCAG436950.030.6257086
CATGGGG109700.029.764674
AACGCAT3800.029.737566
ATGGGGG63350.029.38555
ACGCATA3950.028.6082867
ATCAACG485150.027.4726893
TCAACGC491600.027.3058074
ACGCAGA490850.027.226367
CGCAGAG491400.027.207998
TATCAAC492100.027.1678542
CAACGCA498350.027.0194975
GGGTTAG11350.026.2282031
TATCACG3200.026.018992
CATGGGT53050.026.0098554
GCAGAGT515950.025.8903249
ACATGGG405950.023.9101853
GTACATG432950.023.4054071
TACATGG435500.023.2288952
AGTACTT225650.023.2205812-13
Produced by FastQC (version 0.11.5)