FastQCFastQC Report
Fri 10 Feb 2017
ERR1148384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879186
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245411.3059377836999637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153590.8173219681287536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110940.5903619971626013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53810.2863473865812112No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA40450.21525277433952786No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34310.18257905284522127No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32890.17502258956803637No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA31420.16720005364024634No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29810.15863251429076206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.140326715929131No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC22400.11920054747108588No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21700.11547553036261446No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA20520.10919621580833404No Hit
GGTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAA20250.1077594234950665No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA19590.10424726450707913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1750.044.1808052
AACGCAG310350.034.0956346
ACGCATA1953.8198777E-1133.55317
CTAACGC1102.0450358E-532.443913
ATGGGGG44950.032.287525
GTATCAA329850.032.1245081
CATGGGG73900.031.0682964
ACGCAGA354300.029.8325827
CGCAGAG355800.029.6900968
TACAACG2850.029.2153782
TATCAAC364850.029.0157642
TGGGGGG37950.028.8390276
ATCAACG367400.028.7041133
GCAGAGT369250.028.576419
TCAACGC373850.028.495274
CAACGCA377700.028.2205585
AACGCAT2404.3837645E-1027.2618946
GGTATCA232400.026.8446771
CATGGGT19000.025.044424
GTACAAC5300.024.7104021
Produced by FastQC (version 0.11.5)