FastQCFastQC Report
Fri 10 Feb 2017
ERR1148382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600330
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295250.820063716381554No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA166050.46120772262542603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165270.45904125455166606No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT152480.42351673318834665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137140.3809095277377351No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG74750.20761985706865763No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67200.1866495571239303No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA66300.18414978626959197No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC65730.18256659806184433No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG64090.17801146006060556No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT53530.1486808153697022No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC53230.1478475584182561No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG49650.13790402546433245No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC47680.13243230481650292No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC45750.1270716850955329No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT39180.10882335785886293No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37230.10340718767446316No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA36140.10037968741754227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3350.046.1595952
CATGGGG115950.032.573214
ATGGGGG71500.031.943525
GGGTTAG12600.030.2354871
TGGGGGG59500.028.389586
GTTAGAG15700.028.0342983
GGTTAGA14950.026.2564622
CATGGGT44300.024.3014644
ACGCATA2951.8189894E-1024.1945087
ATCACGC7350.023.4675753
TTAGAGC18050.023.0663264
TCACGCA7450.022.3542124
TAGAGCA17450.022.155195
AGTACTT235500.022.14915812-13
GAGTACT226450.021.6291712-13
ACATGGG545500.021.2398663
GTACACT41450.021.110591
AACGCAG627850.020.8886456
GTAAGTA24850.020.8221872
GTATCAA625700.020.815591
Produced by FastQC (version 0.11.5)